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Related Experiment Videos

Recombineering: a powerful new tool for mouse functional genomics.

N G Copeland, N A Jenkins, D L Court

    Nature Reviews. Genetics
    |October 5, 2001
    PubMed
    Summary

    Recombinogenic engineering (recombineering) uses phage-based systems for efficient bacterial artificial chromosome modification. This advanced genetic engineering accelerates the creation of transgenic mouse models and facilitates complex genomic studies.

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    Rapid engineering of bacterial artificial chromosomes using oligonucleotides.

    Genesis (New York, N.Y. : 2000)·2001

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biotechnology

    Background:

    • Traditional methods for modifying bacterial artificial chromosomes (BACs) are often time-consuming and require restriction enzymes and DNA ligases.
    • Advancements in genetic engineering are crucial for accelerating research in functional genomics and creating sophisticated animal models.

    Purpose of the Study:

    • To introduce and evaluate a novel phage-based homologous recombination system for bacterial artificial chromosome engineering.
    • To demonstrate the efficiency and utility of recombinogenic engineering (recombineering) in modifying genomic DNA.

    Main Methods:

    • Utilized highly efficient phage-based Escherichia coli homologous recombination systems.
    • Performed modification and subcloning of genomic DNA within bacterial artificial chromosomes.

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  • Avoided the use of restriction enzymes and DNA ligases in the engineering process.

    • Main Results:

    • Demonstrated efficient modification and subcloning of genomic DNA in BACs using recombineering.
    • Significantly reduced the time required for creating transgenic mouse models compared to traditional methods.
    • Enabled a wider range of genomic experiments previously considered difficult.

    Conclusions:

    • Recombineering offers a powerful and efficient approach for bacterial artificial chromosome engineering.
    • This technology accelerates the development of transgenic mouse models and enhances functional genomic studies.
    • Recombineering promises to refine genetic analysis of the mouse genome and improve the creation of relevant research models.