Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crystallographic and modeling studies of RNase III suggest a mechanism for double-stranded RNA cleavage.

Structure (London, England : 1993)·2001
Same author

High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.

Genomics·2001
Same author

Bacteriophage lambda: alive and well and still doing its thing.

Current opinion in microbiology·2001
Same author

Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans.

Gene·2001
Same author

Rapid engineering of bacterial artificial chromosomes using oligonucleotides.

Genesis (New York, N.Y. : 2000)·2001

Related Experiment Video

Updated: Jul 26, 2026

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

Recombineering: a powerful new tool for mouse functional genomics.

N G Copeland, N A Jenkins, D L Court

    Nature Reviews. Genetics
    |October 5, 2001
    PubMed
    Summary

    Recombinogenic engineering (recombineering) uses phage-based systems for efficient bacterial artificial chromosome modification. This advanced genetic engineering accelerates the creation of transgenic mouse models and facilitates complex genomic studies.

    More Related Videos

    Generation of Genetically Modified Mice through the Microinjection of Oocytes
    10:19

    Generation of Genetically Modified Mice through the Microinjection of Oocytes

    Published on: June 15, 2017

    Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
    08:01

    Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

    Published on: November 20, 2018

    Related Experiment Videos

    Last Updated: Jul 26, 2026

    Recombineering Homologous Recombination Constructs in Drosophila
    14:23

    Recombineering Homologous Recombination Constructs in Drosophila

    Published on: July 13, 2013

    Generation of Genetically Modified Mice through the Microinjection of Oocytes
    10:19

    Generation of Genetically Modified Mice through the Microinjection of Oocytes

    Published on: June 15, 2017

    Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
    08:01

    Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

    Published on: November 20, 2018

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biotechnology

    Background:

    • Traditional methods for modifying bacterial artificial chromosomes (BACs) are often time-consuming and require restriction enzymes and DNA ligases.
    • Advancements in genetic engineering are crucial for accelerating research in functional genomics and creating sophisticated animal models.

    Purpose of the Study:

    • To introduce and evaluate a novel phage-based homologous recombination system for bacterial artificial chromosome engineering.
    • To demonstrate the efficiency and utility of recombinogenic engineering (recombineering) in modifying genomic DNA.

    Main Methods:

    • Utilized highly efficient phage-based Escherichia coli homologous recombination systems.
    • Performed modification and subcloning of genomic DNA within bacterial artificial chromosomes.
    • Avoided the use of restriction enzymes and DNA ligases in the engineering process.

    Main Results:

    • Demonstrated efficient modification and subcloning of genomic DNA in BACs using recombineering.
    • Significantly reduced the time required for creating transgenic mouse models compared to traditional methods.
    • Enabled a wider range of genomic experiments previously considered difficult.

    Conclusions:

    • Recombineering offers a powerful and efficient approach for bacterial artificial chromosome engineering.
    • This technology accelerates the development of transgenic mouse models and enhances functional genomic studies.
    • Recombineering promises to refine genetic analysis of the mouse genome and improve the creation of relevant research models.