Related Experiment Video
Updated: Oct 4, 2026

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
Published on: May 16, 2021
From hit to lead. Analyzing structure-profile relationships
R Poulain1, D Horvath, B Bonnet
1Department of Chemistry, CEREP, 1 rue du Pr. Calmette, F-59000 Lille, France. rpoulain@phare.univ-lille2.fr
Abstract:
Two compounds, obtained by random screening, and displaying micromolar activities on the mu opiate receptor were used as starting points for optimization. In that work, the traditional concept of the activity of a compound (related to one or a few targets) was extended to the comprehensive pharmacological profile of that compound on more than 70 receptors, transporters, and channels relevant to a CNS-oriented project. Using the two complementary design strategies based on two similarity concepts described in the previous paper, we have obtained analogues with IC(50) values ranging between 0.9 nM and a few micromolar on the mu receptor and displaying qualitatively different profiles. We discuss here, both on a case-by-case basis and from a statistical standpoint, the pharmacological profiles in light of the two similarity concepts.
Related Concept Videos
Structure-Activity Relationships and Drug Design
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence its...
Fischer Projections
Ligand Binding and Linkage
Ligand Binding and Linkage
Resonance and Hybrid Structures
Extraction: Advanced Methods

