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Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
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Catecholamines in murine bone marrow derived mast cells.

J G Freeman1, J J Ryan, C P Shelburne

  • 1Department of Biology, Virginia Commonwealth University, Richmond, VA 23284-2012, USA.

Journal of Neuroimmunology
|October 5, 2001
PubMed
Summary

Mast cells store dopamine and can release it upon activation. High dopamine levels induce apoptosis, a process potentially mediated by mitochondrial pathways and preventable with ascorbic acid.

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Area of Science:

  • Immunology
  • Neuroscience
  • Cell Biology

Background:

  • Mast cells are immune cells involved in allergic responses.
  • Mast cells have been shown to express receptors for neurotransmitters.
  • The role of catecholamines within mast cells is not fully understood.

Purpose of the Study:

  • To investigate the presence and function of catecholamines in mast cells.
  • To determine the effects of exogenous dopamine on mast cell proliferation and survival.

Main Methods:

  • Cultured murine bone marrow-derived mast cells (BMMC) were utilized.
  • Catecholamine levels were measured.
  • Inhibitors of catecholamine synthesis and receptor antagonists were employed.
  • Cell proliferation and apoptosis assays were performed.

Main Results:

  • BMMC store significant amounts of dopamine and can produce norepinephrine and epinephrine.
  • Dopamine release was stimulated by ionomycin activation.
  • Low concentrations of exogenous dopamine did not affect IL-3-induced proliferation.
  • High concentrations of exogenous dopamine reduced proliferation and increased apoptosis, an effect mitigated by ascorbic acid.

Conclusions:

  • Mast cells are a source of dopamine with functional implications.
  • High dopamine levels can induce apoptosis in mast cells via a mitochondrial pathway.
  • Ascorbic acid may protect mast cells from dopamine-induced apoptosis.