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Updated: Jul 14, 2026

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High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
Regional mapping panels for human chromosomes 1, 2, and 7
J C Leonard1, L H Toji, P K Bender
1NIGMS Human Genetic Cell Repository, Coriell Cell Repositories, Coriell Institute for Medical Research, Camden, New Jersey 08103, USA.
Somatic Cell and Molecular Genetics
|October 6, 2001
Summary
The NIGMS Human Genetic Cell Repository created new regional mapping panels for human chromosomes 1, 2, and 7 using somatic cell hybrids. These panels provide valuable resources for genetic mapping and research.
Area of Science:
- Genetics
- Genomics
- Cell Biology
Background:
- The National Institute of General Medical Sciences (NIGMS) Human Genetic Cell Repository collects and distributes human genetic materials.
- Regional mapping panels are essential tools for localizing genes on chromosomes.
Purpose of the Study:
- To assemble and validate regional mapping panels for human chromosomes 1, 2, and 7.
- To provide researchers with new resources for human genetic mapping.
Main Methods:
- Human rodent somatic cell hybrids from 14 laboratories were collected and characterized.
- Each hybrid was verified to contain a stable, defined human chromosomal segment.
- Panels were designed to define 8-10 intervals per chromosome.
Main Results:
- Regional mapping panels for human chromosomes 1, 2, and 7 have been established.
- The chromosome 2 panel is a novel resource, while panels for chromosomes 1 and 7 complement existing ones.
- These panels consist of cell cultures or DNA derived from verified somatic cell hybrids.
Conclusions:
- The NIGMS Repository has successfully created and validated new regional mapping panels for human chromosomes.
- These panels serve as critical resources for precise genetic mapping and research.
- The Repository offers these panels, along with information on numerous other chromosome panels, via its electronic catalog.
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