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Direct sizing of RNA fragments using RNase-generated standards

B Sollner-Webb1, J Cruz-Reyes, L N Rusché

  • 1Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Methods in Enzymology
|October 6, 2001
PubMed
Summary

No abstract available in PubMed .

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...