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Measurement of pyruvate in blood by high-performance liquid chromatography with fluorescence detection
R W Wulkan1, R Verwers, M Neele
1Department of Clinical Biochemistry, Medisch Centrum Rijnmond-Zuid, Rotterdam, The Netherlands. r.wulkan@zuiderziekenhuis.nl
Annals of Clinical Biochemistry
|October 6, 2001
Abstract:
Accurate measurement of pyruvate is particularly important in the detection of mitochondrial enzyme disorders. describe a simple high-performance liquid chromatography assay for pyruvate with derivatization and fluorescence detection. Between-run variation is below 4.5% and the method is linear between I and 1,200 micromol/L. The mean recovery is 101%. After sample pretreatment, the calculated pyruvate concentration proved to be stable for 15 days when samples were kept at -20 degrees C.