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Study of different coupling agents in the conjugation of a V3-based synthetic MAP to carrier proteins

L J Cruz1, E Iglesias, J C Aguilar

  • 1División de Química-Física, Centro de Ingeniería Genética y Biotecnologia, Cubanacán, Habana, Cuba. ljcruz@cigb.edu.cu

Journal of Peptide Science : an Official Publication of the European Peptide Society
|October 6, 2001
PubMed

The conjugation of synthetic peptides to carrier proteins is a widely used method for immunological studies. Different coupling agents have been described to form the conjugate with carrier proteins. In this paper, we demonstrate that the antibody response toward V3-based synthetic MAPs derived from HIV-1, JY1 isolate, conjugated to two different carrier proteins using either m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) or beta-maleimidopropionic acid N-hydroxysuccinimide ester (MPS), or succinic anhydride (SA) show different behaviors. An excellent anti-JY1 response without a strong response to the coupling agent is observed in the case of succinic anhydride spacer. In contrast, MBS produces total abrogation of the antibody response with a high response toward the coupling agent.

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