Jove
Visualize
Contact Us

Related Experiment Videos

Rapid chiral on-chip separation with simplified amperometric detection.

M A Schwarz1, P C Hauser

  • 1The University of Basel, Department of Chemistry, Switzerland.

Journal of Chromatography. A
|October 6, 2001
PubMed
Summary

This study demonstrates a novel capillary electrophoresis method for separating enantiomers of key adrenergic compounds. The technique utilizes a unique amperometric detector and chiral selectors for rapid and sensitive chiral separations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MIF-CD74 signaling impedes microglial M1 polarization and facilitates brain tumorigenesis.

Oncogene·2016
Same author

Methods for retrovirus-mediated gene transfer to fetal lung.

Methods in molecular medicine·2014
Same author

Definition of K(trans) and FA thresholds for better assessment of experimental glioma using high-field MRI: a feasibility study.

Clinical neuroradiology·2013
Same author

Capillary electrophoretic determination of different classes of organic ions by potentiometric detection with coated-wire ion-selective electrodes.

Analytical chemistry·2011
Same author

Potentiometric detection of alkali and alkaline Earth metal cations in capillary electrophoresis with simplified electrode alignment and enhanced separation and sensitivity.

Analytical chemistry·2011
Same author

Acute lung injury: cellular mechanisms and derangements.

Paediatric respiratory reviews·2005
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Electrochemistry

Background:

  • Chiral separation of enantiomers is crucial in pharmaceutical analysis.
  • Traditional methods can be time-consuming and complex.
  • Development of rapid and sensitive chiral separation techniques is ongoing.

Purpose of the Study:

  • To develop a rapid and sensitive capillary electrophoresis method for separating enantiomers of adrenaline, noradrenaline, ephedrine, and pseudoephedrine.
  • To evaluate a novel two-electrode amperometric detector for enhanced detection.
  • To optimize chiral separation using cyclodextrin and crown ether additives.

Main Methods:

  • Capillary electrophoresis on a micromachined device.
  • Amperometric detection with a two-electrode system.

Related Experiment Videos

  • Chiral selectors: carboxymethyl-beta-cyclodextrin and 18-crown-6.

    • Main Results:

    • Successful separation of enantiomers of adrenaline, noradrenaline, ephedrine, and pseudoephedrine.
    • Achieved high plate numbers (up to 20,000) and chiral resolutions (2.5).
    • Obtained detection limits in the order of 10(-7) M with separations completed in under 3 minutes.

    Conclusions:

    • The developed capillary electrophoresis method offers a rapid, sensitive, and efficient approach for chiral separations.
    • The novel two-electrode amperometric detector simplifies the setup without compromising performance.
    • This technique holds promise for enantiomeric purity analysis in pharmaceutical and chemical industries.