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Related Concept Videos

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Prokaryotes

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Related Experiment Video

Updated: Jul 12, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Replication dynamics of the yeast genome.

M K Raghuraman¹, E A Winzeler, D Collingwood

¹Department of Genetics, Department of Mathematics, University of Washington, Seattle, WA 98195, USA. raghu@u.washington.edu

Science (New York, N.Y.)

|October 6, 2001

Summary

This study maps chromosome replication in budding yeast using oligonucleotide microarrays, revealing continuous origin activation during S phase and variable replication fork speeds across the genome. The findings offer insights into DNA replication dynamics and are applicable to other species.

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Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

Area of Science:

Molecular Biology
Genomics
Cell Biology

Background:

Understanding DNA replication is crucial for cell division and genome stability.
The budding yeast Saccharomyces cerevisiae is a model organism for studying eukaryotic DNA replication.

Purpose of the Study:

To map the detailed topography of chromosome replication in Saccharomyces cerevisiae.
To determine the timing of replication for thousands of genomic sites.

Main Methods:

Oligonucleotide microarrays were employed to analyze DNA replication.
Replicated and unreplicated DNA samples from different S phase time points were hybridized to microarrays.

Main Results:

Origin activations occur continuously throughout S phase, with a peak near mid-S phase.
Replication fork movement rates exhibit significant regional variation across the genome.
Replication timing of the two ends of each chromosome is highly correlated.

Conclusions:

The study provides a high-resolution map of DNA replication in Saccharomyces cerevisiae.
The microarray approach is adaptable for studying chromosome replication in other organisms, including humans.