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Continuous beds with vancomycin as chiral stationary phase for capillary electrochromatography
O Kornysova1, P K Owens, A Maruska
1Department of General and Biological Chemistry, Vytautas Magnus University, Kaunas, Lithuania.
Electrophoresis
|October 9, 2001
Summary
This study presents a novel continuous-bed chiral stationary phase (CSP) for capillary electrochromatography (CEC) using vancomycin. The method achieves strong electroosmotic flow (EOF) and high-resolution enantiomeric separations, notably for thalidomide.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Chiral separations are crucial in pharmaceuticals.
- Capillary electrochromatography (CEC) offers high efficiency for enantiomeric separations.
- Developing robust chiral stationary phases (CSPs) is essential for CEC.
Purpose of the Study:
- To develop and characterize a continuous-bed chiral stationary phase (CSP) for capillary electrochromatography (CEC).
- To evaluate the performance of the vancomycin-based CSP in terms of electroosmotic flow (EOF) and enantioselectivity.
Main Methods:
- Preparation of continuous beds in fused silica capillaries via in situ copolymerization.
- Immobilization of vancomycin onto the continuous bed using reductive amination to create the CSP.
- CEC experiments were conducted in both nonaqueous polar organic and aqueous reversed-phase modes.
Main Results:
- The vancomycin-based CSP generated a strong electroosmotic flow (EOF) in both tested modes.
- Successful enantiomeric separation of four racemic compounds was achieved.
- High resolution (Rs = 2.5) and efficiency (120,000 plates/meter) were obtained for thalidomide separation within 10 minutes.
Conclusions:
- The developed continuous-bed vancomycin CSP is effective for high-performance enantiomeric separations in CEC.
- The strong EOF generated by the phase is advantageous for CEC applications.
- This method shows promise for the analysis of chiral compounds, including pharmaceuticals like thalidomide.