Thin-layer gel chromatography of dyed proteins

Area of Science:

• Analytical Chemistry
• Chromatography
• Spectroscopy

Background:

• Plasma chromatography is effective for ultratrace qualitative and quantitative detection of organic compounds.
• It is particularly suitable for analyzing gas chromatographic effluents.
• Reference mobility spectra for various compound classes have been previously established.

Purpose of the Study:

• To present reference mobility spectra for lysergic acid diethylamide (LSD), delta9-tetrahydrocannabinol (delta9-THC), digitoxigenin, and other significant biochemical compounds.
• To demonstrate the utility of plasma chromatography for identifying these specific compounds.

Main Methods:

• Utilized plasma chromatography for the detection and identification of organic compounds.
• Generated positive and negative mobility spectra for reference compounds.
• Investigated the optimal sample quantity range for detection (10^-6 to 10^-12 g).

Main Results:

• Established reference mobility spectra for LSD, delta9-THC, digitoxigenin, and other biochemicals.
• Demonstrated that LSD and delta9-THC can be detected and identified in mixtures at levels of 10^-7 g or less using positive mobility spectra.
• Observed strong MH+ ions and other ions (e.g., (M)NO+, (M)2H+) for all investigated compounds.
• Found that none of the studied compounds exhibited negative mobility spectra.

Conclusions:

• Plasma chromatography is a powerful tool for the ultratrace analysis of complex organic molecules.
• The generated reference spectra facilitate the identification of compounds like LSD and delta9-THC in complex mixtures.
• Positive ion mode plasma chromatography is effective for the detection of the investigated compounds, with no observed negative ion spectra.