Nucleotide sequence analysis of pRS2 and pRS3, two small cryptic plasmids from Oenococcus oeni
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Updated: Oct 4, 2026

Long-Read Plasmid Sequencing and Assembly Using Nanopore Sequencing-Based Workflows
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J M Mesas1, M C Rodríguez, M T Alegre
1Departamento de Química Analítica, Nutrición y Bromatología (Tecnología de los Alimentos), Escuela Politécnica Superior, Universidad de Santiago de Compostela, Lugo, Spain.
Abstract:
Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two subfamilies, one represented by pLo13 and pRS3, the other represented by pOg32, pRS1, and pRS2.
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