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Related Concept Videos

Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
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Related Experiment Video

Updated: Jul 27, 2026

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
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RXR beta isoforms in neuroblastoma cells and evidence for a novel 3'-end transcript.

B Rana1, A D Pearson, C P Redfern

  • 1Department of Endocrinology, Medical Molecualr Biology Group, Medical School, University of Newcastle upon Tyne, UK.

FEBS Letters
|October 10, 2001
PubMed
Summary

Retinoid X receptor beta (RXR beta) expression in neuroblastoma cells is complex, involving multiple isoforms. A novel RXR beta transcript, abundant in various cell types, suggests significant roles in gene regulation.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Retinoid X receptor beta (RXR beta) plays a key role in retinoid signaling pathways.
  • Neuroblastoma cells, particularly SH-SY5Y and SH-SEP, are models for studying retinoid responses.
  • Understanding RXR beta isoforms is crucial for elucidating its function in these cells.

Purpose of the Study:

  • To identify and characterize RXR beta isoforms in neuroblastoma cells.
  • To investigate a putative novel RXR beta transcript.
  • To explore the complexity of RXR beta expression patterns.

Main Methods:

  • RNase protection assays were employed to detect and quantify RXR beta transcripts.
  • Analysis of gene expression patterns in neuroblastoma and other cell types.
  • Investigating transcript structure, including promoter usage and alternative splicing.

Main Results:

  • RXR beta 1 and RXR beta 2 isoforms were identified in neuroblastoma cells.
  • A previously described isoform, RXR beta(SLSR), was expressed at significant levels.
  • A novel, abundant RXR beta transcript was discovered, originating from an internal promoter and exhibiting alternative splicing with retained intron 6.

Conclusions:

  • RXR beta expression in neuroblastoma is more complex than previously understood.
  • The novel RXR beta transcript is widespread and potentially plays a significant role in RXR beta function and regulation.
  • Further research is warranted to fully understand the implications of this complex expression pattern.