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Is interleukin-3 important in antiphospholipid antibody-mediated pregnancy failure?
L W Chamley1, B Konarkowska, A M Duncalf
1Department of Obstetrics and Gynaecology, University of Auckland, National Women's Hospital, Epsom, Auckland, New Zealand. l.chamley@auckland.ac.nz
Objective:
To investigate the effect of interleukin-3 (IL-3) on trophoblast proliferation and expression of beta2-glycoprotein I.
Design:
In vitro cell culture using primary trophoblasts and the cell lines Jeg-3, Jar, and BeWo.
Setting:
Department of Obstetrics and Gynaecology, University of Auckland.
Patient(S):
Women with normal pregnancies.
Intervention(S):
Increasing amounts of IL-3 were added to cultures of primary human trophoblasts, cell lines, or cells treated with a proliferation inhibiting antiphospholipid-like antibody. RNA was extracted from primary human trophoblasts or cell lines.
Main Outcome Measure(S):
We examined basal and IL-3-stimulated cellular proliferation by [3H] thymidine incorporation assay and secretion of beta2-glycoprotein I into culture medium by semiquantitative immunoblot analysis. Reverse transcriptase-polymerase chain reaction analysis was used to demonstrate the presence of IL-3 receptor transcripts.
Result(S):
The IL-3 treatment did not induce proliferation of highly purified primary trophoblast cultures or cell lines but did induce proliferation of contaminating CD45+ cells in trophoblast cultures. The IL-3 did not overcome the antiproliferative effect of an antiphospholipid-like monoclonal antibody on trophoblast. Secretion of beta2-glycoprotein I by trophoblast cultures was time dependent but unaltered by IL-3 treatment.
Conclusion(S):
Our results question the proposed importance of IL-3 in antiphospholipid antibody-mediated fetal death.