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Repeated freezing and thawing does not generally alter assay results for several commonly studied reproductive
R Reyna1, K D Traynor, G Hines
1Department of Obstetrics and Gynecology, The University of Alabama at Birmingham, Birmingham, Alabama 35233-7333, USA.
Objective:
To determine the effect of repeated freezing and thawing, and storage temperature, on the assay results of commonly measured reproductive hormones, and sex hormone-binding globulin (SHBG) in human serum.
Design:
Prospective laboratory study.
Setting:
Academic medical center.
Patient(S):
Four men and three pregnant women in the third trimester.
Intervention(S):
Pooled serum from men and pregnant females were frozen at either -20 degrees C or -70 degrees C. Aliquots were then subjected to repeat freeze/thaw cycles, from 1 to 10 times, and assays were performed after the final freeze/thaw cycle.
Main Outcome Measure(S):
Assay results for eight hormones (FSH, LH, PRL, androstenedione (A), 17alpha-hydroxyprogesterone, P, insulin, and SHBG, as a function of the number of freeze/thaw cycles and storage temperature.
Result(S):
Only SHBG in male serum at -20 degrees C and P in pregnant serum at -70 degrees C showed statistically significant decreases in assay results with repeated freeze/thaw cycles (3.3% and 1.1% per cycle, respectively). All other analytes did not show significant changes as a function of freeze/thaw cycles or storage temperature.
Conclusion(S):
There is no consistent or predictable alteration in the results of SHBG, or the glycoprotein and steroid hormones evaluated, as a function of repeated freeze/thaw or the storage temperature of human serum.
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