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Related Experiment Videos

Mouse BAC ends quality assessment and sequence analyses.

S Zhao1, S Shatsman, B Ayodeji

  • 1The Institute for Genomic Research, Rockville, Maryland 20850, USA. szhao@tigr.org

Genome Research
|October 10, 2001
PubMed
Summary
This summary is machine-generated.

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This study generated over 449,000 mouse BAC end sequences (mBESs), creating a valuable resource for mouse genome research. The high-quality data provides extensive genome coverage and aids in comparative genomics studies.

Area of Science:

  • Genomics
  • Bioinformatics

Background:

  • The mouse genome is a critical model for human biology and disease.
  • Large-scale sequencing projects are essential for comprehensive genome annotation and marker development.

Purpose of the Study:

  • To generate a large, high-quality dataset of mouse BAC end sequences (mBESs).
  • To provide a valuable resource for the scientific community for mouse genome research and comparative genomics.

Main Methods:

  • Utilized a large-scale BAC end-sequencing project at TIGR.
  • Employed ABI3700 capillary sequencers for high-throughput sequencing.
  • Generated 449,234 nonredundant mBESs from RPCI-23 and RPCI-24 libraries.

Main Results:

  • Achieved >80% sequencing success rate with an average read length of 485 bp.

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  • Generated 218 Mb of sequence data, providing 15x clone coverage and 7% sequence coverage.
  • Found significant L1 repeat content and high-quality bases (Q20 length 406 bp) in mBESs.
  • Observed conservation of over 70% of mBES-EST matches between mouse, human, and rat.

    • Conclusions:

    • The generated mBESs represent a significant advancement in mouse genome sequencing.
    • This high-quality dataset will facilitate gene mapping, genome assembly, and comparative genomic studies.
    • The data is a valuable resource for researchers studying mouse and human biology.