Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A case of toxic shock syndrome secondary to severe bone marrow suppression induced by azathioprine in a patient with autoimmune hepatitis].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2026
Same author

[Epidemic characteristics and trend analysis of major injuries deaths among children and adolescents in Jiangsu Province from 2012 to 2021].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2024
Same author

HEIDI: an experiment-management platform enabling high-throughput fragment and compound screening.

Acta crystallographica. Section D, Structural biology·2024
Same author

Application of machine learning techniques in the diagnostic approach of PTSD using MRI neuroimaging data: A systematic review.

Heliyon·2024
Same author

[Investigation of familial tendency of endometriosis].

Zhonghua fu chan ke za zhi·2023
Same author

Associations and relative risks of pulmonary hypertension and lung diseases in individuals with methamphetamine use disorder.

Pulmonology·2023

Related Experiment Video

Updated: Jul 26, 2026

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
10:59

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Published on: November 18, 2013

Valpha gene replacement in a TCRalpha knock-in mouse.

R Golub1, C Y Huang, O Kanagawa

  • 1Department of Immunology, University of Toronto, Toronto, Canada.

European Journal of Immunology
|October 10, 2001
PubMed
Summary

T-cell receptor (TCR) alpha gene replacement was observed in mice, generating functional TCR alpha chain transcripts. This discovery suggests V-gene replacement can diversify the T cell repertoire and the immune system.

More Related Videos

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

Related Experiment Videos

Last Updated: Jul 26, 2026

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
10:59

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Published on: November 18, 2013

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • The T cell receptor (TCR) is crucial for adaptive immunity.
  • V(D)J recombination is the primary mechanism for generating TCR diversity.
  • The potential for V-gene replacement in the TCR alpha locus was previously unexplored.

Purpose of the Study:

  • To investigate the occurrence and function of V-gene replacement in the TCR alpha locus.
  • To determine if V-gene replacement can generate functional TCR alpha chain transcripts.
  • To assess the potential of V-gene replacement in shaping the peripheral T cell repertoire.

Main Methods:

  • Utilized a TCR alpha chain knock-in mouse model.
  • Analyzed splenic T cells for functional TCR alpha chain transcripts.
  • Investigated V-gene replacement events at the Valpha-embedded heptamer site.

Main Results:

  • Demonstrated that V-gene replacement operates within the TCR alpha locus.
  • Identified functional TCR alpha chain transcripts resulting from Valpha-gene replacement.
  • Confirmed Valpha-gene replacement occurs at the Valpha-embedded heptamer site.

Conclusions:

  • TCR alpha V-gene replacement is a viable mechanism for generating TCR diversity.
  • This process can contribute to shaping the peripheral T cell repertoire.
  • The conserved heptamer suggests V-gene replacement is a fundamental immune diversification mechanism shared by B and T cells.