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Updated: Aug 5, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Cell signalling - the proteomics of it all
K Ashman1, M F Moran, F Sicheri
11 Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Canada, M5G 1X5.
Understanding cellular behavior requires mapping global protein interactions. Current methods face technical hurdles, but future advances promise a comprehensive protein interaction map for research.
Area of Science:
- Biochemistry
- Molecular Biology
- Systems Biology
Background:
- Understanding cellular behavior necessitates a global view of molecular interactions.
- DNA microarrays have advanced gene expression profiling, but studying protein interactions presents greater challenges.
- Global protein interaction mapping is crucial for deciphering complex cellular signaling pathways.
Purpose of the Study:
- To review current methodologies for large-scale protein interaction studies.
- To identify technical challenges in global protein interaction analysis.
- To discuss future directions for creating a comprehensive protein interaction map.
Main Methods:
- Review of existing techniques for protein complex isolation and composition determination.
- Discussion of software and database requirements for high-throughput data analysis.
- Analysis of challenges in large-scale, high-throughput proteomic studies.
Main Results:
- Current methods for global protein interaction studies face significant technical hurdles.
- Challenges span protein complex isolation, composition analysis, and data management.
- The development of advanced technologies and interdisciplinary collaboration is essential.
Conclusions:
- A global protein interaction map is vital for biomedical research.
- Overcoming technical and data analysis challenges is key to achieving this goal.
- Future advancements and collaborations will enable the creation of this essential resource.
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