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Enzyme-linked immunoelectrotransfer blotting analysis of human serologic responses to infective hookworm larval
1Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025, China.
Objective:
To explore the possibility of using specific antigens for immunodiagnosis of hookworm disease in endemic area.
Method:
Infective third-stage larvae of the canine hookworm, Ancylostoma caninum (A. caninum), were prepared as the source of antigen. Enzyme-linked immunoelectrotransfer blotting (EITB) was employed as an immunodiagnostic method.
Results:
Two immunodominant bands of hookworm antigens (42 kDa and 55 kDa) were recognized by the sera of hookworm-infected patients (serum dilution 1:200; antigen centrifuged at 36,000 r/m for 20 minutes, but not by sera from negative controls.
Conclusion:
The 42 kDa and 55 kDa A. caninum antigens might be the specific antigens that could be used for immunodiagnosis of hookworm disease in endemic area.