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Aberrant expression of growth factor Wnt-5A in six urinary malignant cell lines
1Department of Urology, First Hospital, Beijing Medical University, Institute of Urology, Beijing Medical University, Beijing 100034, China.
Objective:
To investigate the expression of growth factor Wnt-5A in urinary tumor cell lines.
Methods:
By semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction), the expression of Wnt-5A in six urinary malignant cell lines, one primary cultured renal fibroblast and one case of normal human renal tissue was detected using Gel Doc 1000 computer controlled molecular analysis system.
Results:
The expression of Wnt-5A in-urinary tumor cell lines was much higher than that in primary cultured renal fibroblasts (PRF) and normal human renal tissue (NRT). The levels of Wnt-5A mRNA were different between six malignant cell lines.
Conclusion:
The overexpression of growth factor Wnt-5A has a potential effect on the oncogenesis of urinary malignancies.