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Updated: Oct 4, 2026
A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
Published on: June 26, 2020
Antibody response to E2 glycoprotein induced in mice by immunization with plasmid DNA containing sequence derived
1Institute of Hepatology, People's Hospital, Beijing Medical University, Beijing 100044, China.
Objective:
To induce antibody response against E2 glycoprotein derived from a Chinese genotype III/2a isolate of hepatitis C virus (HCV) in BALB/C mice by plasmid DNA-based immunization.
Methods:
Plasmid p3W14 containing E2/NS1 gene derived from a Chinese genotype III/2a isolate of HCV, which was verified to express 70 kDa E2 glycoprotein in NIH3T3 cells, was used for direct intramuscular injection in BALB/C mice. Specific antibody to E2 glycoprotein was detected by recombinant E2 protein.
Results:
Specific anti-E2 antibody could be detected in mice inoculated with p3W14(5/6). Titer of antibody ranged from 1:15 to 1:120.
Conclusion:
Successfully inducing anti-E2 antibody in mice by plasmid DNA-based immunization containing E2/NS1 gene from a Chinese genotype III/2a isolate of HCV is the first time in China.
