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[Histological study and stereologic analysis of membranous versus endochondral onlay bone grafts]
1Plastic Surgery Hospital of Chinese Academy of Medical Sciences, BaDaChu, Beijing 100041.
Objective:
The experimental study was to determine the differences in changes in the onlay bone grafts of different embryonic origin.
Methods:
The study was designed to compare the potential for appositional bone growth in membranous and endochondral onlay grafts in New-Zealand rabbits and a rhesus monkey. Results were assessed with volumetric measurement, histological examinations and stereologic analysis with double-fluorochrome labeling technique.
Results:
For the membranous and endochondral bone grafts, the volumetric retention rate was (78.4 +/- 3.5)% and (56.0 +/- 5.1)% respectively. The mineralization apposition rate (MAR) was (3.6 +/- 0.8) microns/d and (0.92 +/- 0.33) micron/d; the osteoid seam width (OSW) was (14.56 +/- 2.69) microns and (7.38 +/- 2.20) microns; the tetracycline uptake rate(TUA) was (91.22 +/- 2.69)% and (51.28 +/- 4.11)%, respectively.
Conclusion:
1. It was found that the membranous bone graft maintained its volume to a significantly greater extent than the endochondral bone graft; 2. Membranous bone grafts exhibited higher survival rate and greater activity than endochondral bone grafts; 3. Different changes were also found in the two recipient sites. There was more powerful bone regenerative reaction in the recipient bed of the membranous bone graft than the endochondral one.