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Updated: Aug 17, 2026

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
Published on: May 1, 2020
[Changes in TGF-beta 1 and type I, III procollagen gene expression in keloid and hypertrophic scar]
1Department of Burn and Plastic Surgery, Second Affiliated Hospital of Shantou University Medical College, Shantou 515041.
Objective:
The expression of mRNA for TGF-beta 1, type I, III procollagen in keloid and hypertrophic scar was investigated in order to elucidate the pathogenesis of and the difference between keloid and hypertrophic scar.
Method:
Dot-blot hybridization analysis was used to examine the type I, III procollagen and the expression levels of steady-state mRNA. In situ hybridization allowed direct assessment of the distribution of TGF-beta 1 mRNA in the tissue.
Results:
1. Our study indicated that the expression level of mRNA for TGF-beta 1 significantly increased in keloid and hypertrophic scar compared with normal scar and normal skin. 2. In keloid tissues, type I precollagen mRNA expression was selectively increased. However, in hypertrophic scar, type I and III collagen mRNAs expressions were simultaneously increased.
Conclusion:
TGF-beta 1 plays an important role in the pathogenesis of keloid and hypertrophic scarring. It implicates that distinct molecular mechanisms are operative in the development of keloid and hypertrophic scarring.
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