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Killing effect of interleukin-2 and interferon-alpha activated leukemic bone marrow in remission on K562 leukemic
1Institute of Hematology, Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
Objective:
To evaluate the killing effect of interleukin-2 (IL-2) and interferon-alpha (IFN-alpha) activated bone marrow cells on K562 cells.
Methods:
Semi-solid colony and 3H-TdR incorporating method were used.
Results:
Bone marrow from leukemia patients in remission was activated in vitro with IL-2 for 3 days. The activated bone marrow (ABM) displayed killing effects of 0.31-2.30 logs on K562 cells. This killing effect was further increased to 0.30-3.15 logs when IFN-alpha added with IL-2 to the marrow for activation. IL-2 alone or in combination with IFN-alpha showed no inhibition of CFU-GM and K562 cells. Compared with IL-2 or IFN-alpha alone, the combination of the two cytokines could more effectively maintain the killing effect of ABM on leukemic cells.
Conclusions:
IFN-alpha can augment the purging effect of IL-2 ABM and combination of the two cytokines can effectively maintain the cytotoxicity of ABM.