Related Experiment Videos
Transdermal absorption of phytoestrogens
1Department of Chemistry of Natural Compounds, Institute of Chemical Technology, Prague, Czech Republic.
Die Pharmazie
|October 12, 2001
Summary
Transdermal absorption of daidzein and genistein in olive oil showed decreased plasma and urine levels with repeated application. This suggests phytoestrogens may be captured in the skin after multiple topical uses.
Area of Science:
- Pharmacokinetics and Drug Metabolism
- Dermatology and Skin Science
- Phytochemistry and Natural Products
Background:
- Isoflavones, such as daidzein and genistein, are plant-derived compounds with potential health benefits.
- Transdermal delivery offers a route for systemic absorption of various compounds, including phytoestrogens.
- Understanding the in vivo absorption and disposition of topically applied isoflavones is crucial for therapeutic applications.
Purpose of the Study:
- To investigate the in vivo transdermal absorption of daidzein and genistein applied in olive oil.
- To quantify the plasma and urinary concentrations of isoflavones and their metabolites after topical application.
- To assess the impact of repeated transdermal application on isoflavone disposition.
Main Methods:
- In vivo study design involving topical application of isoflavones in olive oil.
- Gas chromatography-mass spectrometry (GC-MS) for quantifying isoflavone and metabolite concentrations.
- Monitoring of isoflavone levels in both plasma and urine samples.
Main Results:
- Genistein exhibited a 3-fold higher plasma concentration compared to daidzein.
- Daidzein showed 2-3 fold higher urinary excretion than genistein.
- Repeated transdermal application led to a significant decrease in both plasma and urinary isoflavone levels.
- Urinary recovery dropped substantially from the first to the second application (daidzein: 15.9% to 1.6%; genistein: 7.7% to 0.7%).
Conclusions:
- Transdermal application of daidzein and genistein results in differential absorption and excretion patterns.
- Repeated topical application significantly reduces the systemic availability and urinary excretion of these isoflavones.
- The findings suggest a potential sequestration or accumulation of daidzein and genistein within the skin following repeated transdermal administration.