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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
[Molecular characterization of autochthonous clinical isolates of Histoplasma capsulatum var. capsulatum using
D Perrotta1, R Abrantes, C Canteros
1Departamento de Micología, INEI ANLIS Dr. C. G. Malbrán, Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina. dperrotta@anlis.gov.ar
Abstract:
The aim of our work was to determine the genotypic relatedness among H. capsulatum isolates from Argentinian patients with or without AIDS and isolates from other sources and geographic origins. We analyzed band patterns of 16 isolates obtained by RAPD-PCR, 11 Argentinian clinical isolates (8 from patients with AIDS and 3 from patients without AIDS), 2 strains from the CDC, 2 isolates from Mexico and one isolate from Uruguay. With the band patterns obtained, we built a dendrogram, which showed 3 major groups. Group I contained 2 isolates from Mexico, group II 1 strain from CDC and group III included 13 isolates, and was divided into 4 subgroups. Subgroup IIIa contained 2 isolates from patients with and without AIDS. Subgroup IIIb included isolates from patients without AIDS from Chaco. Subgroup IIIc contained 7 isolates from Argentina (from Buenos Aires province) and the Uruguayan isolate (6 isolates from patients with AIDS). Subgroup IIId included only one strain from CDC. All the Argentinian and the Uruguayan isolates went into group III, subgroups IIIa, IIIb and IIIc. This is a preliminary study; however, a relatedness of the RAPD-PCR band patterns and the geographic origin was observed. Although there might be a probable association between immunocompromised patients with AIDS and infective capability of some H. capsulatum isolates, further studies with a larger number of isolates from different sources and geographic origins are necessary to confirm these results.
Insights
Genotyping of Histoplasma capsulatum isolates revealed geographic relatedness among Argentinian strains. Preliminary findings suggest potential links between certain isolates and patient immunocompromise, warranting further investigation.
Area of Science:
- Mycology
- Genetics
- Infectious Diseases
Background:
- Histoplasma capsulatum is a fungal pathogen causing histoplasmosis.
- Understanding genotypic diversity is crucial for epidemiological studies.
Purpose of the Study:
- To determine genotypic relatedness among H. capsulatum isolates.
- To compare Argentinian isolates with those from other regions and sources.
- To explore potential associations with patient immune status.
Main Methods:
- Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) was used.
- Band patterns of 16 H. capsulatum isolates were analyzed.
- A dendrogram was constructed to visualize genetic relationships.
Main Results:
- Three major genetic groups were identified among the isolates.
- Argentinian and Uruguayan isolates predominantly clustered in Group III.
- Geographic origin showed a correlation with RAPD-PCR band patterns.
Conclusions:
- A degree of genotypic relatedness based on geographic origin was observed.
- Preliminary evidence suggests a possible association between H. capsulatum isolates and immunocompromised patients.
- Further research with larger sample sizes is needed to confirm findings.
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