Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human endomucin is an endothelial marker.

C Liu1, Z M Shao, L Zhang

  • 1Division of Surgical Oncology, UCLA School of Medicine, Los Angeles, CA 90095, USA.

Biochemical and Biophysical Research Communications
|October 12, 2001
PubMed
Summary

Researchers identified endomucin as a novel endothelial marker. This cell surface protein is upregulated during tumor angiogenesis and may aid in cancer diagnosis and treatment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Application of acupuncture and moxibustion for keeping shape.

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan·1999
Same author

Two-effective-source method for the calculation of in-air output at various source-to-detector distances in wedged fields.

Medical physics·1999
Same author

Follow up of women with simple ovarian cysts detected by transvaginal sonography in the Tokyo metropolitan area.

British journal of obstetrics and gynaecology·1999
Same author

(R)-3,3,3-Trifluoro-2-hydroxy-2-methylpropionamides are orally active inhibitors of pyruvate dehydrogenase kinase.

Journal of medicinal chemistry·1999
Same author

Expression analysis of multidrug resistance associated genes in neuroblastomas.

Oncology reports·1999
Same author

A random survey of the Cryptosporidium parvum genome.

Infection and immunity·1999

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • Novel endothelial markers are crucial for understanding angiogenesis.
  • Potential applications exist in cancer diagnosis and treatment.
  • Tumor angiogenesis is a key process in cancer progression.

Purpose of the Study:

  • To identify novel endothelial markers associated with tumor angiogenesis.
  • To investigate the expression and characteristics of a newly identified marker, endomucin.

Main Methods:

  • Screening of human umbilical vein endothelial cells (HUVECs) with tumor conditioned media.
  • Suppression subtractive hybridization (SSH) to identify differentially expressed genes.
  • Northern and in situ hybridization to determine tissue and cell-specific expression patterns.

Related Experiment Videos

Main Results:

  • Endomucin was identified as a potential cell surface marker upregulated in the studied system.
  • Human endomucin encodes a predicted 261-amino acid, 27.5-kDa protein with transmembrane and glycosylation features.
  • Endomucin expression is predominantly endothelial cell-specific and abundant in highly vascular tissues.
  • Expression increases in proliferating or stimulated endothelial cells, including those exposed to tumor media or angiogenic factors (bFGF, TNFalpha).

Conclusions:

  • Endomucin is a novel endothelial-specific marker.
  • Its upregulation in response to angiogenic stimuli suggests a role in tumor angiogenesis.
  • Endomucin holds potential as a diagnostic or therapeutic target in cancer.