Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Imaging neural activity with temporal and cellular resolution using FISH.

J F Guzowski1, B L McNaughton, C A Barnes

  • 1Arizona Research Laboratories, Division of Neural Systems, Memory, and Aging, Tucson, AZ 85724, USA. john@nsma.arizona.edu

Current Opinion in Neurobiology
|October 12, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic profiling of the middle temporal gyrus reveals differential glial/neuronal dysregulation across Alzheimer's disease and aging.

bioRxiv : the preprint server for biology·2025
Same author

An Integrated Platform for in vivo Electrophysiology in Spatial Cognition Experiments.

bioRxiv : the preprint server for biology·2023
Same author

Two separate, large cohorts reveal potential modifiers of age-associated variation in visual reaction time performance.

NPJ aging and mechanisms of disease·2021
Same author

Smoking is associated with impaired verbal learning and memory performance in women more than men.

Scientific reports·2021
Same author

Evaluation of a commercial loop-mediated isothermal amplification assay, 3M<sup>TM</sup> Molecular Detection Assay 2 - <i>Campylobacter</i>, for the detection of <i>Campylobacter</i> from poultry matrices.

British poultry science·2021
Same author

Neural ensemble reactivation in rapid eye movement and slow-wave sleep coordinate with muscle activity to promote rapid motor skill learning.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2020

Immediate early genes track neural activity. A new method, cellular compartment analysis of temporal activity, precisely maps neuron activation timing and distinct neuronal populations responding to different experiences.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Immediate early genes (IEGs) are crucial markers for neural activity.
  • Traditional methods lack temporal resolution for studying IEG dynamics.
  • Understanding neuron activation requires precise temporal mapping.

Purpose of the Study:

  • To introduce and validate a novel technique for high-resolution temporal analysis of IEG expression.
  • To overcome limitations of existing methods for tracking neural activation.
  • To visualize distinct neuronal populations activated by specific stimuli.

Main Methods:

  • Cellular compartment analysis of temporal activity (CCAT).
  • Sensitive fluorescence in situ hybridization (FISH).
  • Laser scanning confocal microscopy.

Related Experiment Videos

Main Results:

  • CCAT enables tracking of distinct steps in IEG RNA synthesis and processing.
  • The technique provides precise temporal information on individual neuron activation.
  • Visualization of different neuronal populations engaged by two distinct experiences within a single brain is achieved.

Conclusions:

  • CCAT significantly enhances the temporal resolution of neural activity mapping using IEGs.
  • This method allows for detailed analysis of neuronal responses to stimuli.
  • CCAT offers a powerful tool for studying brain function and experience-dependent neuronal plasticity.