Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Targeted somatic mutagenesis in mouse epidermis.

A K Indra1, M Li, J Brocard

  • 1Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Collège de France, Illkirch, France.

Hormone Research
|October 12, 2001
PubMed
Summary

Researchers developed new mouse models for studying gene function in the skin. These models allow for precise genetic modifications in epidermal cells, aiding disease research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gut commensal Bacteroides faecichinchillae, a potential novel candidate of next-generation probiotics targeting type 2 diabetes.

Beneficial microbes·2026
Same author

Ultrasound- and clinical-defined disease activities are associated with altered bone microarchitecture and lower bone mineral density in patients with rheumatoid arthritis.

Aging clinical and experimental research·2025
Same author

Risk factors and efficacy of different intravitreal treatment options for symptomatic focal vitreomacular traction with or without full-thickness macular hole.

International ophthalmology·2025
Same author

Co-delivery of HIV pre-exposure prophylaxis (PrEP) and HIV testing among publicly insured adolescents and young adults (AYA) receiving medication for opioid use disorder (MOUD).

Drug and alcohol dependence·2024
Same author

Predicted essential fatty acid intakes for a group of dairy cows also apply at individual animal level.

Animal : an international journal of animal bioscience·2023
Same author

Immuno-oncology approaches in uveal melanoma: tebentafusp and beyond.

Immuno-oncology technology·2023

Area of Science:

  • Genetics
  • Molecular Biology
  • Dermatology

Background:

  • Gene targeting in mice is crucial for understanding mammalian gene function.
  • Precise control over somatic mutations enhances the study of gene function and disease modeling.

Purpose of the Study:

  • To establish transgenic mouse models for targeted somatic mutations in the epidermis.
  • To enable temporal and cell-type-specific gene manipulation in skin keratinocytes.

Main Methods:

  • Generation of transgenic mice expressing Cre recombinase or tamoxifen-inducible Cre-ER(T2) under the keratin 14 promoter.
  • Analysis of LoxP-flanked DNA segment excision in epidermal keratinocytes.
  • Assessment of tamoxifen-induced recombination in K14-Cre-ER(T2) mice.

Related Experiment Videos

Main Results:

  • Efficient excision of floxed DNA segments in epidermal keratinocytes of K14-Cre mice.
  • Tamoxifen administration induced efficient recombination in basal keratinocytes of K14-Cre-ER(T2) mice.
  • No significant background recombination observed in K14-Cre-ER(T2) mice without tamoxifen.

Conclusions:

  • Established two transgenic mouse lines (K14-Cre and K14-Cre-ER(T2)) for epidermal gene targeting.
  • These models facilitate the analysis of gene function in keratinocytes.
  • Valuable tools for generating animal models of human skin diseases.