Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantification of elastin from the mouse lung during postnatal development.

C Gonçalves1, J Barros, A Honório

  • 1Institute of Histology and Embryology, Faculty of Medicine, University of Coimbra, Portugal. histoem@ci.uc.pt

Experimental Lung Research
|October 13, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing urban air quality in the context of strict traffic restrictions: particulate matter concentrations, chemical composition and sources.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Independent Evaluation of a Commercial AI Software for Incidental Findings of Pulmonary Embolism (IPE) on a Large Hospital Retrospective Dataset.

Radiology research and practice·2025
Same author

Dual-Loaded Chitosan-Based Nanoparticles: A Novel approach for treating polymicrobial osteomyelitis.

International journal of pharmaceutics·2025
Same author

Multicenter Automated Central Vein Sign Detection Performs as Well as Manual Assessment for the Diagnosis of Multiple Sclerosis.

AJNR. American journal of neuroradiology·2024
Same author

Efficacy and safety of enzyme replacement therapy with alglucosidase alfa for the treatment of patients with infantile-onset Pompe disease: a systematic review and metanalysis.

Frontiers in pediatrics·2024
Same author

Unsupervised clustering to differentiate rheumatoid arthritis patients based on proteomic signatures.

Scandinavian journal of rheumatology·2023

This study quantifies elastic fiber development in mouse lungs using image analysis and HPLC. The findings establish a reliable model for investigating factors affecting pulmonary elastic fibers.

Area of Science:

  • Pulmonary research
  • Developmental biology
  • Connective tissue biology

Background:

  • Elastic fibers are crucial for lung alveolization and secondary septal development.
  • Understanding the dynamic changes in pulmonary elastic fibers during lung development is essential.

Purpose of the Study:

  • To quantitatively assess the development of elastic fibers in mouse lungs from birth to adulthood.
  • To establish a reliable methodology for studying pulmonary elastic fiber synthesis and degradation.

Main Methods:

  • Quantitative image analysis of stained mouse lung tissue using a modified Gomori technique.
  • High-performance liquid chromatography (HPLC) for elastin quantification in mouse lung tissue.
  • Correlation analysis of data obtained from image analysis and HPLC.

Related Experiment Videos

Main Results:

  • A significant positive linear correlation was observed between image analysis and HPLC quantification of elastic fibers.
  • The study successfully characterized the normal establishment of the lung's elastic network during postnatal development.
  • The developed methodology provides a robust model for future research.

Conclusions:

  • The combined approach of staining, image analysis, and HPLC offers a validated method for studying pulmonary elastic fiber development.
  • This model is suitable for investigating the impact of external agents on the synthesis and degradation of lung elastic fibers.