Mother-to-infant transmission of TT virus in Japan

The TT virus (TTV) was detected for the first time by Nishizawa and Okamoto et al. in 1997 in the serum of a patient with post-transfusion hepatitis of unknown origin (non-A-non-G type). TTV was subsequently, also found in the serum of blood donors with no history of blood transfusion, although at a lower rate than among donors with a history of blood transfusion. In the present study, we determined the percentage of TTV carriers among pregnant women with no history of blood transfusion, and evaluated the possibility of mother-child transmission.

Blood was sampled from 300 normal pregnant women with no history of blood transfusion, 10 infants born by vaginal delivery from TTV-positive women, 10 infants born by abdominal cesarean section from TTV-positive women at both 5 days and 3 months after birth, and 10 infants born from TTV-positive women at 6 months after birth. Amniotic fluid and breast milk were sampled from 10 and 30 TTV-positive women, respectively. Informed consent was obtained from all women before sampling. TTV DNA was detected by the nested polymerase chain reaction (PCR) method.

(1) Of the 300 normal pregnant women with no history of blood transfusion, 60 (20%) were TTV-positive. (2) All infants from TTV-positive mothers were TTV-negative at both 5 days and 3 months after birth, regardless of whether they were born by vaginal delivery or abdominal cesarean section. (3) Of the 10 infants who were born from TTV-positive mothers and examined 6 months after birth, 4 (40%) were TTV-positive. (4) Amniotic fluid from all 10 TTV-positive women was TTV-negative. (5) Breast milk from 7 (23.3%) of the 30 TTV-positive women was TTV-positive.

TTV was detected in 20% of pregnant women with no history of blood transfusion, suggesting that TTV infection can occur through non- blood-mediated routes. The possibility of transfer of TTV into amniotic fluid was ruled out due to its absence in amniotic fluid samples. All infants from TTV-positive women were TTV-negative at both 5 days and 3 months after birth, regardless of whether they were born by vaginal delivery or abdominal cesarean section, suggesting that infection in the parturient canal or the pelvis is unlikely. Because TTV was detected in breast milk from TTV-positive women and some of their infants were TTV-positive, breast milk was thought to be a mother-child infection route. These findings suggest that horizontal infection is more likely than vertical infection in mother-child transmission of TTV.