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Target cell of the polycythemia-inducing Friend virus: studies with myleran
Abstract:
A single injection of Myleran reduced the pluripotent hematopoietic stem cell, i.e., colony forming unit(s) (CFU), and the erythropoietin-responsive cell (ERC) in polycythemic mice to around 0.5% that of the controls. Repeated injections of erythropoietin (EP) restored ERC populations, whereas the CFU remained at very low levels. This selective action of Myleran and EP in polycythemic mice seemed to be a good approach for the study of oncogenic action of Friend virus on target cells. When the CFU and ERC compartments were decreased, practically no response to the virus was obtained. When there was an appreciable ERC population present with decreased CFU, leukemogenesis still occurred (as judged by the increased spleen weight). This result was in proportion to the dose of EP, i.e., stimulation of the ERC or closely related cells.
Insights
Myleran treatment significantly reduced hematopoietic stem cells (CFU) and erythropoietin-responsive cells (ERC) in mice. Erythropoietin (EP) restored ERCs but not CFUs, influencing Friend virus leukemogenesis.
Area of Science:
- Hematology
- Virology
- Oncology
Background:
- Polycythemic mice are susceptible to Friend virus-induced leukemogenesis.
- Hematopoietic stem cell (CFU) and erythropoietin-responsive cell (ERC) populations are crucial in this process.
Purpose of the Study:
- To investigate the selective effects of Myleran and erythropoietin (EP) on hematopoietic stem cells (CFU) and erythropoietin-responsive cells (ERC).
- To determine the role of these cell populations in Friend virus-induced leukemogenesis.
Main Methods:
- Administration of Myleran to induce cell depletion in polycythemic mice.
- Treatment with erythropoietin (EP) to restore specific cell populations.
- Infection with Friend virus to assess leukemogenesis.
Main Results:
- Myleran injection drastically reduced both CFU and ERC populations.
- Repeated EP injections restored ERCs but not CFUs.
- Leukemogenesis occurred when ERCs were present with depleted CFUs, correlating with EP dosage.
Conclusions:
- Myleran and EP exhibit selective actions on hematopoietic stem cell compartments.
- The presence of an appreciable ERC population, even with depleted CFUs, facilitates Friend virus leukemogenesis.
- This model provides insights into the oncogenic mechanisms of Friend virus on specific target cells.