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[Determination of resibufogenin and cinobufagin in venenum Bufonis by HPLC]
Abstract
Objective:
To establish a HPLC method to determine the contents of active constituents in Venenum Bufonis.
Method:
In determining the contents of resibufogenin and cinobufagin in the traditional Chinese medicine Venenum Bufonis, the mobile phase was acetonitrile-0.5% KH2PO4 solution(50:50)(pH adjusted with value phosphoric acid to 3.25 +/- 0.02).
Result:
The constituents thus determined have good linearity and separation. The average recovery of resibufogenin was 100.35%, RSD 1.86%; the average recovery of cinobufagin was 100.38%, RSD 2.09%.
Conclusion:
The method was convenient, rapid, accurate and practicable.