Related Experiment Video
Updated: Oct 4, 2026
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
[Investigation on computative spectrophotometry employed in the assay of peaonol in cortex moutan]
1Guangxi Wuzhou Prefectural Institute for Drug Control, Wuzhou 543001.
Objective:
To investigate the easibility of employed computative spectrophotometry in the assay of traditional Chinese medicines.
Method:
Make contents of peaonol in 10 samples of Cortex Moutan were determined by three-wavelength spectrophotometry and dual wavelength K-ratio spectrophotometry, and then compared with the method in Chinese Pharmacopeia.
Result:
The differences of the UV spectrum of different lots of samples bring about relative error. sometimes up to 10%-30% for computative spectrophotometry.
Conclusion:
Computative spectrophotometry should be used carefully in assaying traditional Chinese medicines.
