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Updated: Aug 19, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Published on: May 22, 2014
Activation of human monocyte cell line U937 via cell surface calreticulin
J H Cho1, K J Homma, S Kanegasaki
1Natori Special Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan. natori@postman.riken.go.gp
Abstract:
U937 cells were found to be activated by an antibacterial peptide, KLKLLLLLKLK-NH2 (L5), to generate superoxide anion (O2-)-like peripheral neutrophils. However, the state of cell surface calreticulin, a possible receptor for L5, was suggested to differ between neutrophils and U937 cells. Unlike the former, the latter ones were activated by anti-C-domain peptide antibody of calreticulin even in the absence of L5 and generated O2- in a GTP-binding protein (G-protein)-dependent manner.
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