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Solid-phase extraction and GC-MS analysis of THC-COOH method optimized for a high-throughput forensic drug-testing

P R Stout¹, C K Horn, K L Klette

¹Navy Drug Screening Laboratory, Naval Air Station, Jacksonville, Florida 32212, USA.

Journal of Analytical Toxicology

|October 16, 2001

Summary

A new method for analyzing delta9-tetrahydrocannabinol (THC) in urine samples offers rapid, reliable confirmation. This approach significantly reduces lab processing time, waste, and exposure risks for personnel.

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Area of Science:

Forensic Toxicology
Analytical Chemistry
Pharmacology

Background:

Accurate confirmation of delta9-tetrahydrocannabinol (THC) in numerous urine samples is crucial.
Existing methods may be time-consuming or generate significant waste.

Purpose of the Study:

To develop an efficient extraction, derivatization, and GC-MS method for THC confirmation in urine.
To improve laboratory workflow and safety in forensic toxicology.

Main Methods:

Utilized anion-exchange solid-phase extraction with positive pressure manifold.
Employed rapid derivatization with pentafluoropropionic anhydride/pentafluoropropanol.
Analyzed samples using Gas Chromatography-Mass Spectrometry (GC-MS).

Main Results:

Achieved average recoveries of 95% and a limit of detection of 0.875 ng/mL.
Demonstrated linearity up to 900 ng/mL THC-COOH using a deuterated internal standard.
Validated the method on over 1000 human urine samples, confirming excellent agreement with prior results and identifying an improved qualifier ion (m/z 622).

Conclusions:

The developed method provides a highly efficient and accurate approach for THC confirmation in urine.
Significant reductions in processing time, waste, and personnel exposure were achieved.
The method enhances laboratory safety and throughput for forensic drug testing.