Related Experiment Video
Updated: Oct 4, 2026

Determination of Total Lipid and Lipid Classes in Marine Samples
Published on: December 11, 2021
Composition of total, neutral, and phospholipids in Mapará (Hypophthalmus sp.) from the Brazilian Amazonian area
A J Inhamuns1, M R Bueno Franco
1Departamento de Ciências Pesqueiras, FCA/Universidade do Amazonas, 69070-000, Manaus, AM, Brazil.
Abstract:
Ten lots of mapará (Hypophthalmus sp.), captured from the Amazon River, Brazil, were analyzed for their lipid content and fatty acid composition. This knowledge would allow for the development of adequate processing methods and the formulation of therapeutic diets. Separation into neutral and phospholipids was accomplished by silica-gel column chromatography. Fat from the muscular tissue and from the orbital cavity of the mapará was analyzed by high-resolution gas chromatography-mass spectrometry in two different seasonal periods. There were high levels of saturated and monounsaturated fatty acids in the total and neutral lipid with the principal components 16:0, 18:1omega9, 18:0, 16:1omega7, 14:0, 18:3omega3, and 18:1omega7 in both seasons. In the phospholipids there was a high level of polyunsaturated fatty acids, including primarily 16:0, 18:1omega9, 18:0, 16:1omega7, 22:6omega3, 20:4omega6, 18:3omega3, and 20:5omega3. The ratio omega3/omega6 was the same in the muscular tissue and in the orbital cavity, in both seasonal periods. The muscle tissue could be used in diets that need high levels of polyunsaturated fatty acids, but use of the head to produce an omega3 fatty-acid-rich oil still requires greater study with respect to its economic viability.
Related Concept Videos
Membrane Lipids
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
Asymmetric Lipid Bilayer
Lipids as Anchors
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
Membrane Fluidity
Overview of Fatty Acid Metabolism
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Biosynthesis of Lipids
