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Related Experiment Videos

Structural characterization of two CD1A allelic variants.

M Oteo1, P Arribas, F Setién

  • 1Inmunología, Facultad de Medicina, Universidad Complutense, 28040, Madrid, Spain.

Human Immunology
|October 16, 2001
PubMed
Summary

A novel human CD1A allele, differing by two amino acids, was structurally characterized. This variant, found globally with varying frequencies, is efficiently expressed on cell surfaces, impacting T lymphocyte interactions.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • CD1 molecules present lipid antigens to T lymphocytes and are related to MHC molecules.
  • A previously identified human CD1A allele differs from the wild type by a Cysteine to Tryptophan substitution at position 52.
  • This allele's frequency varies significantly across different ethnic populations.

Purpose of the Study:

  • To structurally characterize the novel human CD1A allele.
  • To investigate the molecular differences between the wild type and the new CD1A allele.
  • To assess the cell surface expression of the variant CD1A protein.

Main Methods:

  • Cloning and sequencing of the full-length cDNA encoding the new CD1A allele.
  • Transfection of wild type and mutant CD1A cDNAs into cell lines (C1R and L721.221).

Related Experiment Videos

  • Analysis of cell surface protein expression using flow cytometry and immunoprecipitation with CD1a-specific antibodies.

    • Main Results:

    • The new CD1A allele encodes a protein with amino acid differences at positions 14 (Threonine vs. Isoleucine) and 52 (Cysteine vs. Tryptophan) compared to the wild type.
    • Both wild type and variant CD1A allelic products were found to be efficiently expressed on the surface of transfected cells.
    • The structural characterization provides insights into CD1a molecule variations and their potential functional implications.

    Conclusions:

    • The novel human CD1A allele is structurally defined by two amino acid substitutions.
    • Efficient cell surface expression of both wild type and variant CD1A molecules was confirmed.
    • Further research can explore the functional consequences of these CD1A variations in immune responses.