Related Experiment Videos
Identification of heme oxygenase in human endometrium
1Comprehensive Reproductive Medicine, Regulation of Internal Environment and Reproduction, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan. n.yoshiki.gyne@med.tmd.ac.jp
Abstract:
The aim of the present study was to investigate the presence of heme oxygenase (HO)-1 and HO-2 in human endometrium at various stages of the menstrual cycle using RT-PCR, Western blotting, and immunohistochemistry. RT-PCR detected mRNA for HO-1 and HO-2 in human endometrium at all stages of the menstrual cycle. Western blotting also revealed the expression of the two distinct HO proteins throughout the menstrual cycle. HO-1 was constitutively expressed, whereas HO-2 expression was apparently greater in the secretory phase than in the menstrual and proliferative phases. Immunohistochemistry showed that distribution of the two HO isoforms had distinct topographic patterns: HO-1 was observed in endometrial epithelial cells and macrophages, whereas HO-2 was found in endothelial cells and smooth muscle cells of blood vessels in the endometrium. The detection of mRNA and protein for HO-1 and HO-2 in normal human endometrium suggests that the carbon monoxide/HO system may play a role in the local control of endometrial function.