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[Vascular endothelial growth factor gene therapy for peripheral artery disease: experimental study]
1Institute of Cardiovascular Research, Beijing Medical University, Beijing 100083.
Objective:
To Study experimentally VEGF gene for treatment of peripheral artery disease.
Methods:
The human VEGF cDNA was cloned into the eukaryotic expression vector pcD2. Using gene suture, the recombinant plasmid was transferred into the hindlimbs' adductor of Rhesus monkey, of which the distal end of the external iliac arteries were ligated and the femoral arteries were completely excised. With angiography, the biological effect of VEGF gene in experimental animals was investigated. Safety tests were analyzed by transferring of VEGF into mouse, rat, and Rhesus monkey, evaluated by biochemistry analysis, histopathological examination, PCR, and indirect ELISA.
Results:
The transfer of VEGF gene stimulated the formation of focal microvessels, established collateral circulation, and augmented blood perfusion. The system had no adverse effect and remarkable pathological change in mouse, rat, and Rhesus monkey.
Conclusion:
The experimental studies indicated that VEGF would be effective and safe for clinical trial after approval.