Related Experiment Videos
[Immune activation genes expression in CD28-costimulated lymphocytes and its application as a diagnostic tool in
1Center of Renal Transplantation, Shanghai First People's Hospital, Shanghai 200080.
Objective:
To investigate the detection of immune activation genes expression as an indicator for the diagnosis of immunological rejection in renal transplantation.
Methods:
In 61 renal allograft recipients, the immune activation genes expression of CD28-costimulated peripheral blood mononuclear cells (PBMC) was studied by RT-PCR.
Results:
The mRNA expression positive rate of IL-10, CD40L, Fas, Perforin, IL-2, IFN-gamma, FasL, bcl-xL, Granzyme B (GB), TGF-beta 1 and IL-4 was 91.8%, 70.5%, 88.5%, 96.7%, 41%, 54.1%, 60.7%, 67.2%, 67.2%, 54.1% and 32.8% respectively. IL-2, IFN-gamma, FasL and Granzyme B were screened out as indicators for the diagnosis of allograft rejection. Any three or more of IL-2, IFN-gamma, FasL, and Granzyme B genes expression could be used as a diagnostic tool for rejection with much favorable sensitivity (93%) and specificity (75%).
Conclusion:
The detection of IL-2, IFN-gamma, FasL and Granzyme B genes expression is beneficial for the evaluation of immunological state in renal allograft recipients. This non-invasive method can be used for clinical diagnosis of allograft rejection.