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Rapid expression of vascular endothelial growth factor in early acute myocardial ischemia in rats
1Department of Pathology, ShanTou University Medical College, Shantou 515031, China.
Objective:
To study the expression of vascular endothelial growth factor (VEGF) in different parts of heart during early acute myocardial ischemia.
Methods:
The expression of VEGF in control and study group in rats was performed with the Strept Avidin-Biotin-Peroxidase Complex (SABC) method.
Results:
A rapid expression of VEGF in ischemic areas was observed 1 hour after the ligation of rat left anterior descending (LAD) coronary artery, and in peri-ischemic and non-ischemic areas 3 hours after ischemia. The grade of expression of VEGF increased with the continuation of myocardial ischemia, the positive myocytes of SABC-VEGF staining mainly localized in peri-ischemic areas 5 hours after ischemia. No expression of VEGF in control group was found.
Conclusion:
The expression of VEGF may be up-regulated in rapid and continuous manner with characteristic spatial and temporal kinetic during early acute myocardial ischemia.