Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Cell clone technique for Taxus cuspidata Sieb. et Zuicc].

D Qiu1, R Li, Y Han

  • 1Institute of Forestry, Chinese Academy of Forestry, Beijing 100091.

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
|October 17, 2001
PubMed
Summary

Optimizing the cell clone technique for Japanese yew (Taxus cuspidata) using needle-derived calli resulted in stable cell lines. This method effectively solved the browning issue, achieving a high growth rate.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterizing α-phase variants in titanium alloys via EBSD: Understanding colour indexing challenges.

Micron (Oxford, England : 1993)·2025
Same author

Using ambient audiovisual experiences to reduce the need for sedation in claustrophobic MRI patients.

Radiography (London, England : 1995)·2025
Same author

Characterization of a retroreflector array for 320-GHz interferometer system in Heliotron J.

The Review of scientific instruments·2023
Same author

Development and initial results of 320 GHz interferometer system in Heliotron J.

The Review of scientific instruments·2022
Same author

Three-dimensional dynamics of fluctuations appearing during pellet ablation process around a pellet in a fusion plasma experiment.

Scientific reports·2022
Same author

[SARS-CoV-2 with transcription regulatory sequence motif mutation poses a greater threat].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2022

Area of Science:

  • Plant biotechnology
  • Cell biology
  • Horticultural science

Context:

  • Taxus cuspidata, a valuable conifer, faces challenges in propagation.
  • Developing efficient cell culture techniques is crucial for its sustainable utilization.
  • Needle-derived calli offer a potential source for cell line establishment.

Purpose:

  • To optimize the cell clone technique for Taxus cuspidata using needle-derived calli.
  • To enhance plating efficiency (PE) and establish stable, high-growth cell lines.
  • To resolve the issue of cell browning in Taxus cuspidata cultures.

Summary:

  • The study identified the third generation of suspension culture cells as having the highest plating efficiency (PE).
  • Phytagel and low melting agarose gel showed comparable PE, while solid-solid double layer culture moderately promoted PE.

Related Experiment Videos

  • Stable cell lines with high growth rates were successfully obtained, completely resolving the browning problem.

    • Impact:

    • Successfully established stable Taxus cuspidata cell lines with a growth rate of 0.4 g.FW/g.FW.d.
    • The optimized cell cloning technique overcomes significant challenges in Taxus cell culture, particularly browning.
    • Provides a foundation for large-scale propagation and potential biotechnological applications of Taxus cuspidata.