[Determination of quercetin in changyanning tablets by RP-HPLC].

Area of Science:

• Analytical Chemistry
• Pharmacognosy
• Chromatography

Context:

• Changyannning Tablets are a traditional Chinese medicine formulation.
• Accurate quantification of active pharmaceutical ingredients is crucial for quality control.
• Quercetin is a key flavonoid with potential health benefits.

Purpose:

• To develop and validate a reverse-phase High-Performance Liquid Chromatography (HPLC) method for the quantitative determination of quercetin.
• To establish a reliable analytical technique for assessing the quercetin content in Changyannning Tablets.

Summary:

• A reverse-phase HPLC method was established using a Shim-Pack CLC-ODS column.
• The mobile phase consisted of methanol, water, and phosphoric acid (55:44.6:0.4) at a flow rate of 1.0 ml/min.
• The method achieved an average recovery of 99.7% with a relative standard deviation of 1.01%, indicating high accuracy and precision.

Impact:

• Provides a validated analytical method for quality control of Changyannning Tablets.
• Ensures consistent and reliable quercetin content in pharmaceutical preparations.
• Supports further research and development of quercetin-based therapeutics.