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Quantitative in vitro assessment of drug phototoxicity by a chemiluminescence method
1Department of Dermatology, Huashan Hospital, Shanghai Medical University, Shanghai 200040, China.
Objective:
To establish a test model for phototoxic agents using the method of chemiluminescence.
Methods:
The phototoxicity of pipemidic acid, doxycycline, griseofuvin and chlorpromazine was detected. These agents and distilled water were irradiated with ultraviolet A (UVA) in the presence of nicotinamide adenine dinucleotide reduced (NADH), and the duplicated samples were incubated in the dark as dark controls. Then luminol was added to the test samples, and the chemiluminescent value was counted and calculated.
Results:
Chemiluminescent values of pipemidic acid, doxycycline and griseofuvin were significantly higher than those in controls. The result of linear regression analysis showed that phototoxic intensity was linear correlated with UVA dosage. The regression coefficient for distilled water was 0.56, indicating that the luminescent value (LV) rose slightly after UVA irradiation. For pipemidic acid, griseofuvin and doxycycline, the regression coefficients reached 76.96, 254.33 and 92.61 respectively, significantly increased in comparison with those of negative controls (P < 0.01).
Conclusion:
Phototoxicity of pipemidic acid, doxycycline and griseofuvin can be detected with the method of chemiluminescence.