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[Phospholipase C negatively regulates tyrosine phosphorylation of EGF receptor in A-431 cells]
A L Evdonin1, A N Leonov, N V Tsupkina
1Institute of Cytology RAS, St. Petersburg. evdonin@mail.ru
Tsitologiia
|October 17, 2001
Abstract:
It is known that EGF induces tyrosine phosphorylation and internalization of the EGF receptor in A-431 cells. U73122, an inhibitor of phospholipase C, induces tyrosine phosphorylation of the EGF receptor and its association with phospholipase C still in nonstimulated cells. In U73122 treated cells EGF exerted no effect on these processes. Receptor-mediated endocytosis was not observed in A-431 cells treated with U73122. The reorganization of actin cytoskeleton was detected in U73122 cells.