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Thioglucosidase activity from Sphingobacterium sp. strain OTG1.

G H Meulenbeld1, S Hartmans

  • 1Department of Agrotechnology and Food Sciences, Wageningen University, The Netherlands. gerwin.meulenbeld@imb.ftns.wau.nl

Applied Microbiology and Biotechnology
|October 17, 2001
PubMed
Summary

Researchers discovered a novel thioglucosidase in Sphingobacterium sp. strain OTG1 that effectively hydrolyzes various thioglycosides, including sinigrin, offering potential for new enzymatic applications.

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Area of Science:

  • Microbiology
  • Enzymology
  • Biochemistry

Background:

  • Thioglycosides are sulfur-containing glycosides with diverse biological roles.
  • Enzymes capable of hydrolyzing thioglycosides are of interest for biotechnological applications.
  • Sphingobacterium species are known for their metabolic versatility.

Purpose of the Study:

  • To isolate and characterize microorganisms with novel thioglucoside hydrolase activity.
  • To investigate the substrate specificity of the identified enzyme.
  • To explore the potential of the isolated strain as a biocatalyst.

Main Methods:

  • Enrichment culture of Sphingobacterium sp. strain OTG1 using octylthioglucoside (OTG).
  • Enzymatic assays using cell-free extracts to determine hydrolase activity.

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  • Use of beta-glucosidase inhibitors (ascorbic acid, D-gluconic acid lactone) to differentiate enzyme activities.
  • Testing hydrolysis of various thioglycosides and sinigrin.

    • Main Results:

    • Isolation of Sphingobacterium sp. strain OTG1 exhibiting thioglucoside hydrolase activity.
    • Hydrolysis of OTG into octanethiol and glucose by cell-free extracts.
    • Demonstration of broad substrate specificity for the novel thioglucosidase, including sinigrin.
    • Sinigrin utilization as a growth substrate by the strain, with limitations at higher concentrations.

    Conclusions:

    • Sphingobacterium sp. strain OTG1 possesses a novel thioglucosidase with broad substrate specificity.
    • The enzyme is distinct from typical beta-glucosidases.
    • The strain shows potential for the degradation of thioglycosides and glucosinolates.