Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimization of retroviral vector generation for clinical application.

A J Schilz1, K Kühlcke, A A Fauser

  • 1EUFETS AG, Idar-Oberstein, Germany.

The Journal of Gene Medicine
|October 17, 2001
PubMed
Summary

Optimizing retroviral vector production in multi-tray cell factories (MTCFs) using serum-free conditions significantly increases vector titers. This scalable, GMP-compliant method enhances gene transfer efficiency for hematopoietic stem cell therapies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Secondary malignancies in chronic myeloid leukemia patients after imatinib-based treatment: long-term observation in CML Study IV.

Leukemia·2016
Same author

A controlled randomized study examining the effects of exercise therapy on patients undergoing haematopoietic stem cell transplantation.

Bone marrow transplantation·2009
Same author

A retrospective comparison of autologous and unrelated donor hematopoietic cell transplantation in myelodysplastic syndrome and secondary acute myeloid leukemia: a report on behalf of the Chronic Leukemia Working Party of the European Group for Blood and Marrow Transplantation (EBMT).

Leukemia·2007
Same author

Reduced-toxicity conditioning with fludarabine and treosulfan prior to allogeneic stem cell transplantation in multiple myeloma.

Bone marrow transplantation·2007
Same author

Long-term follow-up of peripheral blood stem cell transplantation from mismatched related and unrelated donors.

Clinical transplantation·2007
Same author

Isolated intraperitoneal relapse of AML after allogeneic hematopoietic stem cell transplantation in a patient with extensive chronic GVHD and full donor chimerism in bone marrow.

Leukemia & lymphoma·2006

Area of Science:

  • Gene Therapy
  • Hematopoietic Stem Cell Biology
  • Bioprocessing Engineering

Background:

  • Gene transfer into hematopoietic cells offers therapeutic potential for blood disorders.
  • Low retroviral vector titers limit clinical gene therapy efficacy.
  • Optimizing Good Manufacturing Practice (GMP) vector production is crucial for clinical success.

Purpose of the Study:

  • To optimize retroviral vector production for clinical gene therapy.
  • To enhance vector titers and transduction efficiency.
  • To develop a scalable, GMP-compliant production protocol.

Main Methods:

  • Established stable retroviral producer clones with single vector integrations.
  • Optimized vector production in multi-tray cell factories (MTCFs).

Related Experiment Videos

  • Investigated effects of harvest medium, cell density, and harvest time.

    • Main Results:

    • Achieved high-titer vector stocks using serum-free medium.
    • Reduced harvest medium volume increased titers up to four-fold.
    • Enabled repetitive harvests over four days at optimal cell densities.
    • Produced large quantities of serum-free vector supernatant in 40-tray MTCFs.
    • Demonstrated high gene transfer efficiency into primary human CD34+ progenitor cells.

    Conclusions:

    • Developed an easy-to-handle, large-scale vector production protocol in MTCFs.
    • Protocol is applicable to various adherent producer cell lines.
    • The method adheres to current GMP guidelines for clinical applications.