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Published on: April 2, 2014
Periapical lesion progression and cytokine expression in an LPS hyporesponsive model
1Department of Endodontology, University of Connecticut Health Center, Farmington 06030-1715, USA. fouad@nso.uchc.edu
Aim:
The purpose of this study was to compare periapical lesion progression and the expression of the bone modulating cytokines IL-1alpha, TNF-alpha, IL-4, IL-6 and IL-11 in periapical lesions of normal and C3H/HeJ (LPS hyporesponsive) mice.
Methodology:
Pulps of both mandibular first molars from C3H/HeJ and BALB/c (normal) mice were exposed and inoculated with normal mouse oral microorganisms for 2, 4, 6, and 8 weeks. After euthanasia, specimens were prepared for histological examination. A quantitative evaluation of the lesional area and immunohistochemical stain counts was performed.
Results:
There were no statistically significant differences in progression of periapical lesions for both mouse strains with time (two-factor ANOVA, P > 0.05). The immunohistochemical staining revealed no overall differences between the two strains in levels of expression of the cytokines (P > 0.05). IL-11 expression did not change from control levels in BALB/c mice, but correlated with the expression of IL-6 and IL-4 in C3H/HeJ mice.
Conclusion:
Responsiveness to LPS may not be significant in the pathogenesis of periapical lesions and in cytokine expression within the lesions, when the lesions are induced by non-specific oral flora.
